Cancer Isoform of a Tumor-Associated Cell Surface NADH Oxidase (tNOX) Has Properties of a Prion

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• 作者：Matthew Kelker ; Chinpal Kim ; Pin-Ju Chueh ; Rodney Guimont ; Dorothy M. Morré ; and D. James Morré
• 刊名：Biochemistry
• 出版年：2001
• 出版时间：June 26, 2001
• 年：2001
• 卷：40
• 期：25
• 页码：7351 - 7354
• 全文大小：68K
• 年卷期：v.40,no.25(June 26, 2001)
• ISSN：1520-4995

文摘

We have described a drug-responsive form of a cell surface NADH oxidase (hydroquinoneoxidase) of cancer cells (tNOX) that exhibits unusual characteristics including resistance to proteases,resistance to cyanogen bromide digestion, and an ability to form amyloid filaments closely resemblingthose of spongiform encephalopathies and all of which are characteristics of PrP^sc (PrP^res), the presumedinfective and proteinase K resistant particle of the scrapie prion. The tNOX protein from the HeLa cellsurface copurified with authentic glyceraldehyde-3-phosphate dehydrogenase (muscle form) (GAPDH).Surprisingly, the tNOX-associated muscle GAPDH also was proteinase K resistant. In this paper, weshow that combination of authentic rabbit muscle GAPDH with tNOX renders the GAPDH resistant toproteinase K digestion. This property, that of converting the normal form of a protein into a likeness ofitself, is one of the defining characteristics of the group of proteins designated as prions.