Tangeretin from Citrus reticulate Inhibits Respiratory Syncytial Virus Replication and Associated Inflammation in Vivo

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• 作者：Jiao-Jiao Xu ; Zhong Liu ; Wei Tang ; Guo-Cai Wang ; Hau Yin Chung ; Qiu-Ying Liu ; Ling Zhuang ; Man-Mei Li ; Yao-Lan Li
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：November 4, 2015
• 年：2015
• 卷：63
• 期：43
• 页码：9520-9527
• 全文大小：491K
• ISSN：1520-5118

文摘

Human respiratory syncytial virus (RSV) is a common pathogen that causes pneumonia and bronchiolitis in infants and young children. Our previous study showed that tangeretin from Citrus reticulate possessed potent in vitro anti-RSV effects comparable to that of ribavirin. Therefore, in this study, we investigated the in vivo anti-RSV activity of tangeretin in 3-week-old male BALB/c mice. A plaque reduction assay and fluorescence quantitative polymerase chain reaction (FQ-PCR) showed that tangeretin inhibited RSV replication in the lung of mice. Moreover, a luminex assay indicated tangeretin relieved RSV-induced lung inflammation by attenuating interleukin (IL)-1尾 secretion. Possible anti-inflammatory mechanisms of tangeretin were preliminarily explored using a RSV-infected macrophage model. A FQ-PCR, enzyme-linked immunosorbent assay (ELISA), and luciferase assay revealed that tangeretin inhibited RSV-induced inflammation by suppressing nuclear factor-魏B (NF-魏B) activation. This study demonstrates that tangeretin inhibited RSV replication and RSV-induced lung inflammation in vivo and may be useful in preventing and treating RSV infections and inflammation.