Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells
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- 作者：Eddie Y. Chung ; Christopher J. Ochs ; Yi Wang ; Lei Lei ; Qin Qin ; Andrew M. Smith ; Alex Y. Strongin ; Roger Kamm ; Ying-Xin Qi ; Shaoying Lu ; Yingxiao Wang
- 刊名：Nano Letters
- 出版年：2015
- 出版时间：August 12, 2015
- 年：2015
- 卷：15
- 期：8
- 页码：5025-5032
- 全文大小：529K
- ISSN：1530-6992
文摘
We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.