Evaluation of Antioxidant Activity of Vetiver (Vetiveria zizanioides L.) Oil and Identification of Its Antioxidant Constituents
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- 作者：Hyun-Jin Kim ; Feng Chen ; Xi Wang ; Hau Yin Chung ; and Zhengyu Jin
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：October 5, 2005
- 年：2005
- 卷：53
- 期：20
- 页码：7691 - 7695
- 全文大小：86K
- 年卷期：v.53,no.20(October 5, 2005)
- ISSN：1520-5118
文摘
Antioxidant capacities of vetiver (Vetiveria zizanioides) oil were evaluated by two different in vitroassays: the DPPH
free radical scavenging assay and the Fe2+-metal chelating assay. Results showedthat the vetiver oil (VO) possessed a strong free radical scavenging activity when compared to standardantioxidants such as butylated hydroxytoluene (BHT) and 
-tocopherol. However, its metal chelatingcapacity was relatively weak. VO (10 
L/mL) dissolved in methanol exhibited ~93% free radicalscavenging activity in the DPPH assay and ~34% Fe2+ chelating activity in the metal chelating assay.By contrast, 10 mM BHT and 0.1 mM -tocopherol exhibited 93 and 89% free radical scavengingactivities in the DPPH assay, respectively, and 1 mM EDTA exhibited ~97% activity in the metalchelating assay. Among the complex constituents in the crude VO, 
-vetivenene, -vetivone, and-vetivone, which had shown strong antioxidant activities, were isolated and identified using variouschromatographic techniques including silica gel open column chromatography, silica HPLC, and GC-MS. These results show that VO and some of its inherent components can be potential alternativenatural antioxidants.Keywords: Vetiver oil; Vetiveria zizanioides; antioxidant activity; DPPH; terpenoids; vetivone
free radical scavenging assay and the Fe2+-metal chelating assay. Results showedthat the vetiver oil (VO) possessed a strong free radical scavenging activity when compared to standardantioxidants such as butylated hydroxytoluene (BHT) and -tocopherol. However, its metal chelatingcapacity was relatively weak. VO (10 L/mL) dissolved in methanol exhibited ~93% free radicalscavenging activity in the DPPH assay and ~34% Fe2+ chelating activity in the metal chelating assay.By contrast, 10 mM BHT and 0.1 mM -tocopherol exhibited 93 and 89% free radical scavengingactivities in the DPPH assay, respectively, and 1 mM EDTA exhibited ~97% activity in the metalchelating assay. Among the complex constituents in the crude VO, -vetivenene, -vetivone, and-vetivone, which had shown strong antioxidant activities, were isolated and identified using variouschromatographic techniques including silica gel open column chromatography, silica HPLC, and GC-MS. These results show that VO and some of its inherent components can be potential alternativenatural antioxidants.Keywords: Vetiver oil; Vetiveria zizanioides; antioxidant activity; DPPH; terpenoids; vetivone