Antioxidant and Quinone Reductase-Inducing Constituents of Black Chokeberry (Aronia melanocarpa) Fruits

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• 作者：Jie Li ; Ye Deng ; Chunhua Yuan ; Li Pan ; Heebyung Chai ; William J. Keller ; A. Douglas Kinghorn
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：November 21, 2012
• 年：2012
• 卷：60
• 期：46
• 页码：11551-11559
• 全文大小：311K
• 年卷期：v.60,no.46(November 21, 2012)
• ISSN：1520-5118

文摘

Using in vitro hydroxyl radical-scavenging and quinone reductase-inducing assays, bioactivity-guided fractionation of an ethyl acetate-soluble extract of the fruits of the botanical dietary supplement, black chokeberry (Aronia melanocarpa), led to the isolation of 27 compounds, including a new depside, ethyl 2-[(3,4-dihydroxybenzoyloxy)-4,6-dihydroxyphenyl] acetate (1), along with 26 known compounds (2鈥?b>27). The structures of the isolated compounds were identified by analysis of their physical and spectroscopic data ([伪]_D, NMR, IR, UV, and MS). Altogether, 17 compounds (1鈥?b>4, 9, 15鈥?b>17, and 19鈥?b>27) showed significant antioxidant activity in the hydroxyl radical-scavenging assay, with hyperin (24, ED₅₀ = 0.17 渭M) being the most potent. The new compound (1, ED₅₀ = 0.44 渭M) also exhibited potent antioxidant activity in this assay. Three constituents of black chokeberry fruits doubled quinone reductase activity at concentrations <20 渭M, namely, protocatechuic acid [9, concentration required to double quinone reductase activity (CD) = 4.3 渭M], neochlorogenic acid methyl ester (22, CD = 6.7 渭M), and quercetin (23, CD = 3.1 渭M).

Keywords:

Aronia melanocarpa; black chokeberry; Rosaceae; ethyl 2-[(3,4-dihydroxybenzoyloxy)-4,6-dihydroxyphenyl] acetate; antioxidant constituents; hydroxyl radical-scavenging activity; quinone reductase-inducing activity