Selective Detection of 5-Formyl-2鈥?deoxycytidine in DNA Using a Fluorogenic Hydroxylamine Reagent

详细信息    查看全文

• 作者：Pu Guo ; Shengyong Yan ; Jianlin Hu ; Xiwen Xing ; Changcheng Wang ; Xiaowei Xu ; Xiaoyu Qiu ; Wen Ma ; Chunjiang Lu ; Xiaocheng Weng ; Xiang Zhou
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：July 5, 2013
• 年：2013
• 卷：15
• 期：13
• 页码：3266-3269
• 全文大小：218K
• 年卷期：v.15,no.13(July 5, 2013)
• ISSN：1523-7052

文摘

Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.