Bioconjugated Nanoparticles for DNA Protection from Cleavage

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• 作者：Xiao-xiao He ; Kemin Wang ; Weihong Tan ; Bin Liu ; Xia Lin ; Chunmei He ; Du Li ; Shasheng Huang ; and Jun Li
• 刊名：Journal of the American Chemical Society
• 出版年：2003
• 出版时间：June 18, 2003
• 年：2003
• 卷：125
• 期：24
• 页码：7168 - 7169
• 全文大小：97K
• 年卷期：v.125,no.24(June 18, 2003)
• ISSN：1520-5126

文摘

We have developed a novel method to protect DNA from cleavage using bioconjugated nanoparticles. Positively charged amino-modified silica nanoparticles have been directly prepared using water-in-oil microemulsion. Plasmid DNA can be easily enriched onto the positively charged nanoparticle surface, and the DNA strands are well protected from enzymatic cleavage. When incubated with nuclease enzyme for enzymatic cleavage, free plasmid DNA strands are completely cleaved, while those on the nanoparticle surfaces are intact. Our results clearly demonstrate unique properties of nanomaterials when combined with biomolecules. Our simple bionanotechnology will be highly useful in DNA separation, manipulation, and detection, and possibly in genetic engineering and gene therapy, as plasmid DNA can be protected in cellular environments without any change in its property.