Synthesis, Characterization, and Utility of Thermoresponsive Natural/Unnatural Product Macroligands for Affinity Chromatography

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• 作者：Min Zhou ; Ananthapadmanab Sivaramakrishnan ; Krishan Ponnamperuma ; Woon-Kai Low ; Chunmei Li ; Jun O. Liu ; David E. Bergbreiter ; Daniel Romo
• 刊名：Organic Letters
• 出版年：2006
• 出版时间：November 9, 2006
• 年：2006
• 卷：8
• 期：23
• 页码：5247 - 5250
• 全文大小：125K
• 年卷期：v.8,no.23(November 9, 2006)
• ISSN：1523-7052

文摘

The synthesis and characterization of thermoresponsive, water-soluble poly-N-isopropyl acrylamide (PNIPAM) derived macroligands displayingcyclosporin A (CsA) and dexamethasone (Dex) for use as novel affinity resins are described. Characterization of these soluble macroligands,including ligand loading and integrity, was determined by ¹H NMR spectroscopy. One of the CsA macroligands was used in a protein affinityexperiment to capture known binding proteins of CsA, the cyclophilins, from Jurkat T-cell lysates.