l-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells

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• 作者：Guoliang Ke ; Chunming Wang ; Yun Ge ; Nanfeng Zheng ; Zhi Zhu ; Chaoyong James Yang
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：November 21, 2012
• 年：2012
• 卷：134
• 期：46
• 页码：18908-18911
• 全文大小：280K
• 年卷期：v.134,no.46(November 21, 2012)
• ISSN：1520-5126

文摘

Noninvasive and accurate measurement of intracellular temperature is of great significance in biology and medicine. This paper describes a safe, stable, and accurate intracellular nano-thermometer based on an l-DNA molecular beacon (l-MB), a dual-labeled hairpin oligonucleotide built from the optical isomer of naturally occurring d-DNA. Relying on the temperature-responsive hairpin structure and the FRET signaling mechanism of MBs, the fluorescence of l-MBs is quenched below the melting temperature and enhanced with increasing temperature. Because of the excellent reversibility and tunable response range, l-MBs are very suitable for temperature sensing. More importantly, the non-natural l-DNA backbone prevents the l-MBs from binding to cellular nucleic acids and proteins as well as from being digested by nucleases inside the cells, thus ensuring excellent stability and accuracy of the nano-thermometer in a complex cellular environment. The l-MB nano-thermometer was used for the photothermal study of Pd nanosheets in living cells, establishing the nano-thermometer as a useful tool for intracellular temperature measurement.