Surface-Mediated Chromate-Resistant Mechanism of Enterobacter Cloacae Bacteria Investigated by Atomic Force Microscopy
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- 作者：Chunpeng Yang ; Yangjian Cheng ; Xiaoyan Ma ; Ying Zhu ; Hoi-Ying Holman ; Zhang Lin ; Chen Wang
- 刊名：Langmuir
- 出版年：2007
- 出版时间：April 10, 2007
- 年：2007
- 卷：23
- 期：8
- 页码：4480 - 4485
- 全文大小：563K
- 年卷期：v.23,no.8(April 10, 2007)
- ISSN：1520-5827
文摘
The Enterobacter cloacae CYS-25 strain isolated from a chromate plant shows a strong capability for chromateresistance instead of chromate reduction in aerobic conditions. In this study, atomic force microscopy (AFM) wasused for studying the morphology characteristics of bacterial properties during the chromate resistance process. Theaverage length of E. cloacae bacteria in the stationary phase is about 2.3 ± 0.6 
m, while under the stimulation of400 mg/L CrO42-, the length of bacteria increases to 3.2 ± 0.7 m. Height and phase images showed that, with theaddition of CrO42-, the smooth surface of bacteria changed into one with discontinuous features with characteristicdimension of 40-200 nm. Analysis reveals that these compact convex patches are organic components stimulatedby CrO42-. A chromate resistance mechanism relating to the overexpression of extracellular biologic components forpreventing the permeability of CrO42- into the cell is proposed as the survival strategy of E. cloacae in chromatesituation.
m, while under the stimulation of400 mg/L CrO42-, the length of bacteria increases to 3.2 ± 0.7 m. Height and phase images showed that, with theaddition of CrO42-, the smooth surface of bacteria changed into one with discontinuous features with characteristicdimension of 40-200 nm. Analysis reveals that these compact convex patches are organic components stimulatedby CrO42-. A chromate resistance mechanism relating to the overexpression of extracellular biologic components forpreventing the permeability of CrO42- into the cell is proposed as the survival strategy of E. cloacae in chromatesituation.