Highly Fluorescent and Specific Molecular Probing of (Homo)Cysteine or Superoxide: Biothiol Detection Confirmed in Living Neuronal Cells

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• 作者：Dhiraj P. Murale ; Hwajin Kim ; Wan Sung Choi ; David G. Churchill
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：July 19, 2013
• 年：2013
• 卷：15
• 期：14
• 页码：3630-3633
• 全文大小：246K
• 年卷期：v.15,no.14(July 19, 2013)
• ISSN：1523-7052

文摘

Chemodosimetric action in detection of cysteine and homocysteine (310- and 290-fold) and superoxide inputs (336-fold increase) gives significant fluorescence intensity increases. Detection limits of 2.13 脳 10^鈥? M, 1.37 脳 10^鈥? M, and 2.71 脳 10^鈥? M, respectively, are biorelevant and are consistent with 鈥淥R鈥?logic gating, demonstrated in intracellular biothiol detection in neuronal cells by way of novel fluorescein derivatization. As per our knowledge, this is the first example of a novel fluorescent probe based on the nucleophilic substitution reaction of biothiols and superoxide through ester cleavage.