Biotinylated Biodegradable Nanotemplated Hydrogel Networks for Cell Interactive Applications
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- 作者：Jason D. Clapper ; Megan E. Pearce ; C. Allan Guymon ; Aliasger K. Salem
- 刊名：Biomacromolecules
- 出版年：2008
- 出版时间：April 2008
- 年：2008
- 卷：9
- 期：4
- 页码：1188 - 1194
- 全文大小：1658K
- 年卷期：v.9,no.4(April 2008)
- ISSN：1526-4602
文摘
We describe the synthesis of a novel biotinylated nanotextured degradable hydrogel that can be rapidly surface engineered with a diverse range of biotinylated moieties. The hydrogel is synthesized by reacting methacrylated biotin-PEG with dimethacrylated PLA-b-PEG-b-PLA (LPLDMA, PEG = poly(ethylene glycol), PLA = poly(lactic acid)),or dimethacrylated PEG-b-PLA-b-PEG (PLPDMA). Methacrylated biotin-PEG is prepared by reacting biotin-PEG-OH with methacrylic anhydride. Biotin-PEG-OH is prepared by reacting α-hydroxy-ω-amine PEG with N-hydroxysuccinimide-biotin. Confirmation of the final product is determined using 1H NMR and Fourier transform infrared spectroscopy (FTIR). The integrity and surface presentation of the biotin units is observed spectrophotometrically using the HABA/avidin assay. To produce nanostructured polymer topography, a self-assembling lyotropic liquid crystalline mesophase is used as a polymerization template, generating biotinylated hydrogels with highly organized lamellar matrix geometry. Traditionally processed isotropic hydrogels are used for comparison. Scanning electron microscopy shows that isotropic hydrogels have a smooth glassy appearance while lamellar templated hydrogels have defined surface topographical features that enhance preosteoblast human palatal mesenchymal cell (HEPM) attachment. Engineering the surfaces of the hydrogels with cell adhesive Arg-Gly-Asp (RGD) peptide sequences using the biotin–avidin interaction significantly enhances cell attachment. Surface engineering of cell adhesive peptides in conjunction with the lamellar template induced surface topography generates additive enhancements in cell attachment.