Distinguishing the Chemical Moiety of Phosphoenolpyruvate That Contributes to Allostery in Muscle Pyruvate Kinase

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• 作者：James M. Urness ; Kelly M. Clapp ; J. Cody Timmons ; Xinyan Bai ; Nalin Chandrasoma ; Keith R. Buszek ; Aron W. Fenton
• 刊名：Biochemistry
• 出版年：2013
• 出版时间：January 8, 2013
• 年：2013
• 卷：52
• 期：1
• 页码：1-3
• 全文大小：201K
• 年卷期：v.52,no.1(January 8, 2013)
• ISSN：1520-4995

文摘

A series of substrate analogues has been used to determine which chemical moieties of the substrate phosphoenolpyruvate (PEP) contribute to the allosteric inhibition of rabbit muscle pyruvate kinase by phenylalanine. Replacing the carboxyl group of the substrate with a methyl alcohol or removing the phosphate altogether greatly reduces substrate affinity. However, removal of the carboxyl group is the only modification tested that removes the ability to allosterically reduce the level of Phe binding. From this, it can be concluded that the carboxyl group of PEP is responsible for energetic coupling with Phe binding in the allosteric sites.