Spectroscopic, Molecular Modeling, and NMR-Spectroscopic Investigation of the Binding Mode of the Natural Alkaloids Berberine and Sanguinarine to Human Telomeric G-Quadruplex DNA
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- 作者：Irene Bessi ; Carla Bazzicalupi ; Christian Richter ; Hendrik R. A. Jonker ; Krishna Saxena ; Claudia Sissi ; Matteo Chioccioli ; Sara Bianco ; Anna Rita Bilia ; Harald Schwalbe ; Paola Gratteri
- 刊名：ACS Chemical Biology
- 出版年：2012
- 出版时间：June 15, 2012
- 年：2012
- 卷：7
- 期：6
- 页码：1109-1119
- 全文大小：600K
- 年卷期：v.7,no.6(June 15, 2012)
- ISSN：1554-8937
文摘
G-quadruplex structures can be formed at the single-stranded overhang of telomeric DNA, and ligands able to stabilize this structure have recently been identified as potential anticancer drugs. Among the potential G-quadruplex binders, we have studied the binding ability of berberine and sanguinarine, two members of the alkaloid family, an important class of natural products long known for medicinal purpose. Our spectroscopic (CD, NMR, and fluorescence) studies and molecular modeling approaches revealed binding modes at ligand鈥揷omplex stoichiometries >1:1 and ligand self-association induced by DNA for the interactions of the natural alkaloids berberine and sanguinarine with the human telomeric G-quadruplex DNA.