Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology

详细信息    查看全文

• 作者：Roberta Bordoni ; Alessandra Mezzelani ; Clarissa Consolandi ; Andrea Frosini ; Ermanno Rizzi ; Bianca Castiglioni ; Claudia Salati ; Nelson Marmiroli ; Rosangela Marchelli ; Luigi Rossi Bernardi ; Cristina Bat
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2004
• 出版时间：March 10, 2004
• 年：2004
• 卷：52
• 期：5
• 页码：1049 - 1054
• 全文大小：101K
• 年卷期：v.52,no.5(March 10, 2004)
• ISSN：1520-5118

文摘

We have applied the ligation detection reaction (LDR) combined with a universal array approach tothe detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene fromBt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5fmol (nearly 60 pg) of PCR amplified transgenic material was inequivocally detected with excellentlinearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility ofextending the LDR/universal array format to detect in parallel several transgenic sequences that arebeing developed for food applications.Keywords: Genetically modified organisms; GMOs; polymerase chain reaction; PCR; ligation detectionreaction; LDR; universal array; Bt-176 transgenic maize