Improved HPLC Determination of Phenolic Compounds in Cv. Golden Delicious Apples Using a Monolithic Column
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- 作者：Fabio Chinnici ; Anna Gaiani ; Nadia Natali ; Claudio Riponi ; and Sergio Galassi
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：January 14, 2004
- 年：2004
- 卷：52
- 期：1
- 页码：3 - 7
- 全文大小：74K
- 年卷期：v.52,no.1(January 14, 2004)
- ISSN：1520-5118
文摘
A rapid HPLC-DAD determination of phenols in apple using an RP monolithic column is reported.Because of the hydrodynamic advantages offered by this kind of column and the use of acidifiedacetonitrile as eluent, assays of apple extracts can be performed in <21 min. Assays of pulp andpeel extracts were carried out without the need for time-consuming sample pretreatment exceptfiltration. Several flavanols, hydroxycinnamic acids, dihydrochalcones, and six quercetin glycosideswere identified and quantified. A seventh quercetin derivative, two chalcone-related compounds, andthree hydroxycinnamic derivatives were also found. Peels proved to be richer in phenols than pulps,the former being composed mainly of (-)-epicatechin, procyanidin B2, chlorogenic acid, phloridzin,hyperin, and avicularin. In pulps, where the chlorogenic acid was the principal phenolic compound,quercetin glycosides were found in very low amounts.Keywords: HPLC-DAD; monolithic column; phenolics; apple; Golden Delicious; analysis