Antifungal Proteins in Commercial Hybrids and Elite Sorghums

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• 作者：Feliciano P. Bejosano ; Ralph D. Waniska ; and William L. Rooney
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2003
• 出版时间：September 24, 2003
• 年：2003
• 卷：51
• 期：20
• 页码：5911 - 5915
• 全文大小：76K
• 年卷期：v.51,no.20(September 24, 2003)
• ISSN：1520-5118

文摘

The antifungal proteins (AFP) in the caryopsis of commercial and elite sorghums grown in severalenvironments were related to grain mold resistance. Previous studies revealed that improved grainmold resistance was correlated with higher levels of AFP at combine harvest maturity [50 days afteranthesis (DAA)] or with better retention of AFP after physiological maturity (30 DAA). Commercialhybrids and public parental lines and hybrids were grown in College Station, TX, during 2000, 2001,and 2002. Samples of caryopses were collected at 30 and 50 DAA, and caryopsis proteins wereextracted, blotted, identified with immunoassays, and quantified for two AFP (chitinase and sormatin).Sorghums varied in amounts of AFP and their ability to retain AFP after physiological maturity. Theenvironment in 2002 was conducive for fungal deterioration of grain, and a wide range of AFP andgrain molding was observed at harvest maturity. High levels of AFP and low levels of grain moldingwere observed in non-mold-conducive environments in 2000 and 2001. Commercial and elitesorghums with higher levels and increased retention of AFP had less grain molding in the mold-conducive environment.Keywords: Chitinase; sormatin; antifungal proteins; grain mold; plant resistance