Supercritical Fluids Are Superior Media for Catalysis by Cross-Linked Enzyme Microcrystals of Subtilisin Carlsberg

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• 作者：Nuno Fontes ; M.Concei&ccedil ; ã ; o Almeida ; Silvia Garcia ; Cé ; lia Peres ; Johann Partridge ; Peter J. Halling ; and Susana Barreiros
• 刊名：Biotechnology Progress
• 出版年：2001
• 出版时间：April 2001
• 年：2001
• 卷：17
• 期：2
• 页码：355 - 358
• 全文大小：49K
• 年卷期：v.17,no.2(April 2001)
• ISSN：1520-6033

文摘

We report on the performance of cross-linked enzyme microcrystals (CLECs) ofsubtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECsin SC-ethane was found to be 2- to 10-fold greater than in hexane under the sameconditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilizedpowders showed much lower activities, reflecting the same hydration hysteresis effectsas in organic solvents. Reaction rates were much lower in SC-CO₂, especially at higherwater activity, probably as a result of acid-base effects of carbonic acid on the enzyme.