Analysis of Multiple Metabolites of Tocopherols and Tocotrienols in Mice and Humans
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- 作者：Yang Zhao ; Mao-Jung Lee ; Connie Cheung ; Ji-Hyeung Ju ; Yu-Kuo Chen ; Ba Liu ; Long-Qin Hu ; Chung S. Yang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：April 28, 2010
- 年：2010
- 卷：58
- 期：8
- 页码：4844-4852
- 全文大小：866K
- 年卷期：v.58,no.8(April 28, 2010)
- ISSN：1520-5118
文摘
Tocopherols and tocotrienols, collectively known as vitamin E, are essential antioxidant nutrients. The biological fates and metabolite profiles of the different forms are not clearly understood. The objective of this study is to simultaneously analyze the metabolites of different tocopherols and tocotrienols in mouse and human samples. Using HPLC/electrochemical detection and mass spectrometry, 18 tocopherol-derived and 24 tocotrienol-derived side-chain degradation metabolites were identified in fecal samples. Short-chain degradation metabolites, in particular γ- and δ-carboxyethyl hydroxychromans (CEHCs) and carboxymethylbutyl hydroxychromans (CMBHCs) were detected in urine, serum, and liver samples, with tocopherols additionally detected in serum and liver samples. The metabolite profiles of tocotrienols and tocopherols were similar, but new tocotrienol metabolites with double bonds were identified. This is the first comprehensive report describing simultaneous analysis of different side-chain metabolites of tocopherols and tocotrienols in mice and humans. Urinary metabolites may serve as useful biomarkers for the nutritional assessment of vitamin E.