Multiphase Oscillatory Flow Strategy for in Situ Measurement and Screening of Partition Coefficients

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• 作者：Milad Abolhasani ; Connor W. Coley ; Klavs F. Jensen
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：November 3, 2015
• 年：2015
• 卷：87
• 期：21
• 页码：11130-11136
• 全文大小：458K
• ISSN：1520-6882

文摘

Taking advantage of the difference between the surface energies of aqueous and organic solvents on a Teflon substrate, a fully automated small-scale strategy is developed on the basis of gas-driven oscillatory motion of a biphasic slug for high-throughput in situ measurement and screening of partition coefficients of organic substances between aqueous and organic phases. The developed oscillatory flow strategy enables single partition coefficient data point measurement within 8 min (including the sample preparation time) which is 360 times faster than the conventional 鈥渟hake-flask鈥?method, while using less than a 30 渭L volume of the two phases and 9 nmol of the target organic substance. The developed multiphase strategy is validated using a conventional shake-flask technique. Finally, the developed strategy is extended to include automated screening of partition coefficients at physiological temperature.