Tb3+-tRNA for LRET Studies of Protein Synthesis

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• 作者：Dulce Alonso ; Wei Liu ; Gabriel Rosenblum ; Tomoyasu Mani ; Yale E. Goldman ; Barry S. Cooperman
• 刊名：Bioconjugate Chemistry
• 出版年：2013
• 出版时间：July 17, 2013
• 年：2013
• 卷：24
• 期：7
• 页码：1186-1190
• 全文大小：254K
• 年卷期：v.24,no.7(July 17, 2013)
• ISSN：1520-4812

文摘

When suitably labeled bulk tRNAs are transfected into cells they give rise to FRET (fluorescence resonance energy transfer) signals via binding to ribosomes that provide a measure of total protein synthesis. Application of this approach to monitoring rates of specific protein synthesis requires achieving a very high signal-to-noise ratio. Such high ratios may be attainable using LRET (luminescence resonance energy transfer) in place of FRET. Lanthanide complexes containing an antenna chromophore are excellent LRET donors. Here we describe the synthesis of a Phe-tRNA<sup>Phesup> labeled with a Tb<sup>3+sup> complex, denoted Tb<sup>3+sup>-Phe-tRNA<sup>Phesup> that, notwithstanding the bulkiness of the Tb<sup>3+sup> complex, is active in protein synthesis.