Fast Hadamard Transform Capillary Electrophoresis for On-Line, Time-Resolved Chemical Monitoring

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• 作者：Kevin L. Braun ; Suminda Hapuarachchi ; Facundo M. Fernandez ; and Craig A. Aspinwall
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：March 1, 2006
• 年：2006
• 卷：78
• 期：5
• 页码：1628 - 1635
• 全文大小：219K
• 年卷期：v.78,no.5(March 1, 2006)
• ISSN：1520-6882

文摘

We report a new approach for collecting and deconvoluting the data in Hadamard transform capillary electrophoresis, referred to as fast Hadamard transform capillaryelectrophoresis (fHTCE). Using fHTCE, total analysistimes can be reduced by up to 48% per multiplexedseparation compared to conventional Hadamard transform capillary electrophoresis (cHTCE) while providingcomparable signal-to-noise ratio enhancements. In fHTCE, the sample is injected following a pseudorandompulsing sequence derived from the first row of a simplexmatrix (S-matrix) in contrast to cHTCE, which utilizes asequence of twice the length. In addition to the temporalsavings provided by fHTCE, a 50% reduction in sampleconsumption is also realized due to the decreased numberof sample injections. We have applied fHTCE to theanalysis of mixtures of neurotransmitters and relatedcompounds to yield improved signal-to-noise ratios witha total analysis time under 10 s. In addition, we demonstrate the capability of fHTCE to perform time-resolvedmonitoring of changes in the concentration of modelneurochemical compounds.