Fluorescent Ag Clusters via a Protein-Directed Approach as a Hg(II) Ion Sensor
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- 作者：Cunlan Guo ; Joseph Irudayaraj
- 刊名：Analytical Chemistry
- 出版年：2011
- 出版时间：April 15, 2011
- 年：2011
- 卷：83
- 期：8
- 页码：2883-2889
- 全文大小：900K
- 年卷期：v.83,no.8(April 15, 2011)
- ISSN：1520-6882
文摘
Proteins have proven to be particularly attractive as effective ligands in the synthesis of nano- and subnanoscaled materials because of their multiple chelating and functional groups imparting unique functionalities. However, protein-directed fluorescent metal cluster synthesis is still a challenge but a promising area of research. Here, we report on the synthesis of new water-soluble, stable, fluorescent Ag clusters via a facile, green method using denatured bovine serum albumin (dBSA) as a stabilizing agent. The dBSA with its 35 free cysteine residues could contribute to polyvalent interactions with the Ag clusters and serve as effective stabilizing agents for these clusters. The as-prepared Ag clusters showed high fluorescence emission at 637 nm and were stable even in 1 M NaCl. The fluorescent Ag clusters were then used in the detection of Hg2+ with high sensitivity and selectivity. The detection limit was 10 nM in the linear range from 10 nM to 5 渭M.