Polyvalent Oligonucleotide Iron Oxide Nanoparticle “Click” Conjugates

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• 作者：Joshua I. Cutler ; Dan Zheng ; Xiaoyang Xu ; David A. Giljohann ; Chad A. Mirkin
• 刊名：Nano Letters
• 出版年：2010
• 出版时间：April 14, 2010
• 年：2010
• 卷：10
• 期：4
• 页码：1477-1480
• 全文大小：263K
• 年卷期：v.10,no.4(April 14, 2010)
• ISSN：1530-6992

文摘

We have utilized the copper-catalyzed azide−alkyne reaction to form a dense monolayer of oligonucleotides on a superparamagnetic nanoparticle core. These particles exhibit the canonical properties of materials densely functionalized with DNA, which can be controlled by modulating the density of oligonucleotides on the surface of the particles. Furthermore, like their Au analogues, these particles can easily cross HeLa (cervical cancer) cell membranes without transfection agents due to their dense DNA shell. Importantly, this approach should be generalizable to other azide-functionalized particles.