A New Approach for Mapping Sialylated N-Glycosites in Serum Proteomes
详细信息 查看全文
- 作者：Bart Ghesquiè ; re ; Lien Buyl ; Hans Demol ; Jozef Van Damme ; An Staes ; Evy Timmerman ; Joë ; l Vandekerckhove ; Kris Gevaert
- 刊名：Journal of Proteome Research
- 出版年：2007
- 出版时间：November 2007
- 年：2007
- 卷：6
- 期：11
- 页码：4304 - 4312
- 全文大小：219K
- 年卷期：v.6,no.11(November 2007)
- ISSN：1535-3907
文摘
A new approach for proteome-wide analysis of sialylated N-glycopeptides based on the diagonalchromatographic COFRADIC technology is presented here. The use of (2-3,6,8,9) neuraminidase iscentral to isolate sialylated N-glycopeptides out of a complex peptide mixture. Two different COFRADICtechniques are introduced here, either without or with post-metabolic oxygen-18 labeling (direct versusindirect sorting), and when applied to immuno-depleted mouse serum, we herewith identified 93sialylated glycosylation sites in 53 serum proteins.