In Vitro Antibacterial Phenolic Extracts from 鈥淪ugarbag鈥?Pot-Honeys of Australian Stingless Bees (Tetragonula carbonaria)
详细信息 查看全文
- 作者：C. Flavia Massaro ; Daniel Shelley ; Tim A. Heard ; Peter Brooks
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2014
- 出版时间：December 17, 2014
- 年：2014
- 卷：62
- 期：50
- 页码：12209-12217
- 全文大小：337K
- ISSN：1520-5118
文摘
Australian stingless bee honeys have been shown to exert antioxidant and in vitro antimicrobial properties; however their bioactive factors remained unidentified. This study investigated the antibacterial properties of phenolic extracts from Tetragonula carbonaria honeys. Honeys were harvested from beehives in three sites of South East Australia. Liquid鈥搇iquid extractions yielded the phenolic concentrates, for analyses by liquid and gas chromatography mass spectrometry. Antibacterial assays were conducted against Staphylococcus aureus and Klebsiella pneumoniae by in vitro agar diffusion and broth dilution assays. The phenolic extracts averaged to 5.87 mg/100 g of raw honeys, and constituents were 3-phenyllactic acid, lumichrome, diglycosylflavonoids, norisoprenoids. The honeys did not contain methylglyoxal, dihydroxyacetone or phenolics characteristic of Leptospermum nectars. Hydrogen peroxide content amounted up to 155.8 渭M in honeys. Beside the bactericidal effects of hydrogen peroxide at 760 渭M, other antibacterial factors were the phenolic extracts of 鈥渟ugarbag鈥?honeys that were active at minimum bactericidal concentrations of 1.2鈥?.8 mg/mL.