Injectable Nano-Network for Glucose-Mediated Insulin Delivery
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- 作者：Zhen Gu ; Alex A. Aimetti ; Qun Wang ; Tram T. Dang ; Yunlong Zhang ; Omid Veiseh ; Hao Cheng ; Robert S. Langer ; Daniel G. Anderson
- 刊名：ACS Nano
- 出版年：2013
- 出版时间：May 28, 2013
- 年：2013
- 卷：7
- 期：5
- 页码：4194-4201
- 全文大小：651K
- 年卷期：v.7,no.5(May 28, 2013)
- ISSN：1936-086X
文摘
Diabetes mellitus, a disorder of glucose regulation, is a global burden affecting 366 million people across the world. An artificial 鈥渃losed-loop鈥?system able to mimic pancreas activity and release insulin in response to glucose level changes has the potential to improve patient compliance and health. Herein we develop a glucose-mediated release strategy for the self-regulated delivery of insulin using an injectable and acid-degradable polymeric network. Formed by electrostatic interaction between oppositely charged dextran nanoparticles loaded with insulin and glucose-specific enzymes, the nanocomposite-based porous architecture can be dissociated and subsequently release insulin in a hyperglycemic state through the catalytic conversion of glucose into gluconic acid. In vitro insulin release can be modulated in a pulsatile profile in response to glucose concentrations. In vivo studies validated that these formulations provided improved glucose control in type 1 diabetic mice subcutaneously administered with a degradable nano-network. A single injection of the developed nano-network facilitated stabilization of the blood glucose levels in the normoglycemic state (<200 mg/dL) for up to 10 days.