Inhomogeneous Quenching as a Limit of the Correlation Between Fluorescence Polarization and Conformation of Single Molecules
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- 作者：Rafael Camacho ; Daniel Thomsson ; Giuseppe Sforazzini ; Harry L. Anderson ; Ivan G. Scheblykin
- 刊名：The Journal of Physical Chemistry Letters
- 出版年：2013
- 出版时间：March 21, 2013
- 年：2013
- 卷：4
- 期：6
- 页码：1053-1058
- 全文大小：337K
- 年卷期：v.4,no.6(March 21, 2013)
- ISSN：1948-7185
文摘
The photophysical properties of conjugated polymers (CPs) largely depend on the interactions between the CP and its environment. We present a study of two polymers with identical conjugated backbones, bare and insulated, that showed different fluorescence excitation modulation depth histograms. However, the polarization differences are not related to differences in conformation, as commonly believed, but to the existence of 鈥渄ark鈥?chromophores in the bare polymer that are statically quenched. This results in inhomogeneous quenching of the polymer chain that breaks the correlation between excitation fluorescence polarization and conjugated polymer chain conformation.