Analysis of Swainsonine and Swainsonine N-Oxide as Trimethylsilyl Derivatives by Liquid Chromatography–Mass Spectrometry and Their Relative Occurrence in Plants Toxic to Livestock

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• 作者：Dale R. Gardner ; Daniel Cook
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：August 10, 2016
• 年：2016
• 卷：64
• 期：31
• 页码：6156-6162
• 全文大小：310K
• 年卷期：0
• ISSN：1520-5118

文摘

There are limited data concerning the occurrence of swainsonine N-oxide in plants known to contain swainsonine and its relative impact on toxicity of the plant material. A liquid chromatography–mass spectrometry method based on a solvent partitioning extraction procedure followed by trimethylsilylation and analysis using reversed phase high-pressure liquid chromatography–mass spectrometry was developed for the analysis of swainsonine and its N-oxide. The concentrations of each were measured in several swainsonine-containing taxa as well as two endophytic isolates that produce swainsonine. In vegetative samples the relative percent of N-oxide to free base ranged from 0.9 to 18%. In seed samples the N-oxide to free base ratio ranged from 0 to 10%. The measured concentrations of swainsonine N-oxide relative to swainsonine only slightly increases the actual toxicity of the various plant samples in a combined assay of both compounds.