Membrane Fusion-Competent Virus-Like Proteoliposomes and Proteinaceous Supported Bilayers Made Directly from Cell Plasma Membranes

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• 作者：Deirdre A. Costello ; Chih-Yun Hsia ; Jean K. Millet ; Teresa Porri ; Susan Daniel
• 刊名：Langmuir
• 出版年：2013
• 出版时间：May 28, 2013
• 年：2013
• 卷：29
• 期：21
• 页码：6409-6419
• 全文大小：520K
• 年卷期：v.29,no.21(May 28, 2013)
• ISSN：1520-5827

文摘

Virus-like particles are useful materials for studying virus鈥揾ost interactions in a safe manner. However, the standard production of pseudovirus based on the vesicular stomatitis virus (VSV) backbone is an intricate procedure that requires trained laboratory personnel. In this work, a new strategy for creating virus-like proteoliposomes (VLPLs) and virus-like supported bilayers (VLSBs) is presented. This strategy uses a cell blebbing technique to induce the formation of nanoscale vesicles from the plasma membrane of BHK cells expressing the hemagglutinin (HA) fusion protein of influenza X-31. These vesicles and supported bilayers contain HA and are used to carry out single particle membrane fusion events, monitored using total internal reflection fluorescence microscopy. The results of these studies show that the VLPLs and VLSBs contain HA proteins that are fully competent to carry out membrane fusion, including the formation of a fusion pore and the release of fluorophores loaded into vesicles. This new strategy for creating spherical and planar geometry virus-like membranes has many potential applications. VLPLs could be used to study fusion proteins of virulent viruses in a safe manner, or they could be used as therapeutic delivery particles to transport beneficial proteins coexpressed in the cells to a target cell. VLSBs could facilitate high throughput screening of antiviral drugs or pathogen鈥揾ost cell interactions.