Wild Blueberry (Vaccinium angustifolium Ait.) Polyphenols Target Fusobacterium nucleatum and the Host Inflammatory Response: Potential Innovative Molecules for Treating Periodontal Disea

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• 作者：Amel Ben Lagha ; St茅phanie Dudonn茅 ; Yves Desjardins ; Daniel Grenier
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 12, 2015
• 年：2015
• 卷：63
• 期：31
• 页码：6999-7008
• 全文大小：456K
• ISSN：1520-5118

文摘

Blueberries contain significant amounts of flavonoids to which a number of beneficial health effects in humans have been associated. The present study investigated the effect of a polyphenol-rich lowbush blueberry (Vaccinium angustifolium Ait.) extract on the two main etiologic components of periodontitis, a multifactorial disorder affecting the supporting structures of the teeth. Phenolic acids, flavonoids (flavonols, anthocyanins, flavan-3-ols), and procyanidins made up 16.6, 12.9, and 2.7% of the blueberry extract, respectively. The blueberry extract showed antibacterial activity (MIC = 1 mg/mL) against the periodontopathogenic bacterium Fusobacterium nucleatum. This property may result from the ability of blueberry polyphenols to chelate iron. Moreover, the blueberry extract at 62.5 渭g/mL inhibited F. nucleatum biofilm formation by 87.5 卤 2.3%. Subsequently, the ability of the blueberry extract to inhibit the NF-魏B signaling pathway in U937-3x魏B cells was investigated. The blueberry extract dose-dependently inhibited the activation of NF-魏B induced by F. nucleatum. In addition, a pretreatment of macrophages with the blueberry extract (62.5 渭g/mL) inhibited the secretion of IL-1尾, TNF-伪, and IL-6 by 87.3 卤 1.3, 80.7 卤 5.6, and 28.2 卤 9.3%, respectively, following a stimulation with F. nucleatum. Similarly, the secretion of MMP-8 and MMP-9 was also dose-dependently inhibited. This dual antibacterial and anti-inflammatory action of lowbush blueberry polyphenols suggests that they may be promising candidates for novel therapeutic agents.