Superparamagnetic Fe3O4 Nanoparticles: Synthesis by Thermal Decomposition of Iron(III) Glucuronate and Application in Magnetic Resonance Imaging

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• 作者：Vitalii Patsula ; Lucie Kosinová ; Marija Lovrić ; Lejla Ferhatovic Hamzić ; Mariia Rabyk ; Rafal Konefal ; Aleksandra Paruzel ; Miroslav Šlouf ; Vít Herynek ; Srećko Gajović ; Daniel Horák
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：March 23, 2016
• 年：2016
• 卷：8
• 期：11
• 页码：7238-7247
• 全文大小：601K
• ISSN：1944-8252

文摘

Monodisperse superparamagnetic Fe₃O₄ nanoparticles coated with oleic acid were prepared by thermal decomposition of Fe(III) glucuronate. The shape, size, and particle size distribution were controlled by varying the reaction parameters, such as the reaction temperature, concentration of the stabilizer, and type of high-boiling-point solvents. Magnetite particles were characterized by transmission electron microscopy (TEM), as well as electron diffraction (SAED), X-ray diffraction (XRD), dynamic light scattering (DLS), and magnetometer measurements. The particle coating was analyzed by atomic absorption spectroscopy (AAS) and attenuated total reflection (ATR) Fourier transform infrared spectroscopy (FTIR) spectroscopy. To make the Fe₃O₄ nanoparticles dispersible in water, the particle surface was modified with α-carboxyl-ω-bis(ethane-2,1-diyl)phosphonic acid-terminated poly(3-O-methacryloyl-α-D-glucopyranose) (PMG–P). For future practical biomedical applications, nontoxicity plays a key role, and the PMG–P&Fe₃O₄ nanoparticles were tested on rat mesenchymal stem cells to determine the particle toxicity and their ability to label the cells. MR relaxometry confirmed that the PMG–P&Fe₃O₄ nanoparticles had high relaxivity but rather low cellular uptake. Nevertheless, the labeled cells still provided visible contrast enhancement in the magnetic resonance image. In addition, the cell viability was not compromised by the nanoparticles. Therefore, the PMG–P&Fe₃O₄ nanoparticles have the potential to be used in biomedical applications, especially as contrast agents for magnetic resonance imaging.