Functionalized Carbon Nanotubes for Detecting Viral Proteins
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- 作者：Yian-Biao Zhang ; Mandakini Kanungo ; Alexander J. Ho ; Paul Freimuth ; Daniel van der Lelie ; Michelle Chen ; Samuel M. Khamis ; Sujit S. Datta ; A. T. Charlie Johnson ; James A. Misewich ; Stanislaus S. Wong
- 刊名：Nano Letters
- 出版年：2007
- 出版时间：October 2007
- 年：2007
- 卷：7
- 期：10
- 页码：3086 - 3091
- 全文大小：443K
- 年卷期：v.7,no.10(October 2007)
- ISSN：1530-6992
文摘
We investigated the biocompatibility, specificity, and activity of a ligand-receptor-protein system covalently bound to oxidized single-walledcarbon nanotubes (SWNTs) as a model proof-of-concept for employing such SWNTs as biosensors. SWNTs were functionalized under ambientconditions with either the Knob protein domain from adenovirus serotype 12 (Ad 12 Knob) or its human cellular receptor, the CAR protein,via diimide-activated amidation. We confirmed the biological activity of Knob protein immobilized on the nanotube surfaces by using itslabeled conjugate antibody and evaluated the activity and specificity of bound CAR on SWNTs, first, in the presence of fluorescently labeledKnob, which interacts specifically with CAR, and second, with a negative control protein, YieF, which is not recognized by biologically activeCAR proteins. In addition, current-gate voltage (I-Vg) measurements on a dozen nanotube devices explored the effect of protein binding onthe intrinsic electronic properties of the SWNTs, and also demonstrated the devices' high sensitivity in detecting protein activity. All datashowed that both Knob and CAR immobilized on SWNT surfaces fully retained their biological activities, suggesting that SWNT-CAR complexescan serve as biosensors for detecting environmental adenoviruses.