Volume Cytometry: Microfluidic Sensor for High-Throughput Screening in Real Time
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- 作者：Daniel A. Ateya ; Frederick Sachs ; Philip A. Gottlieb ; Steve Besch ; and Susan Z. Hua
- 刊名：Analytical Chemistry
- 出版年：2005
- 出版时间：March 1, 2005
- 年：2005
- 卷：77
- 期：5
- 页码：1290 - 1294
- 全文大小：263K
- 年卷期：v.77,no.5(March 1, 2005)
- ISSN：1520-6882
文摘
Regulation of cell volume was one of the earliest evolutionary demands for life and remains a universal measureof cell metabolism. Since conventional methods to measure cell volume, such as microscopy, are complex andtime-consuming, cell volume has not been used as thebasis for cell-based screening. We have developed amicrofabricated chip that can measure the volume ofsmall numbers of cells in real time with unprecedentedresolution. The method is applicable to adherent orsuspended populations of cells and membrane-boundorganelles. Our prototype device can detect volumechanges in a monolayer of tissue-cultured astrocytesresponding to anisotonic stimuli of <1mOsm. We determined the sensitivity to antibiotics of different E. colistrains in <10 min at 24 
C. This time can be reduced athigher temperatures enabling on-site clinical testing ofinfectious agents. Using the chip to screen natural products, we found a peptide in spider venom that inhibitseukaryotic volume regulation at ~100pM. The prototypechip made in silicon is inexpensive, reusable, and runson low-voltage electrical power. The technology can bereadily transferred to large arrays in plastic.
C. This time can be reduced athigher temperatures enabling on-site clinical testing ofinfectious agents. Using the chip to screen natural products, we found a peptide in spider venom that inhibitseukaryotic volume regulation at ~100pM. The prototypechip made in silicon is inexpensive, reusable, and runson low-voltage electrical power. The technology can bereadily transferred to large arrays in plastic.