Separation and Characterization of Amines from Individual Atrial Gland Vesicles of Aplysia californica

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• 作者：Sheri J. Lillard ; Daniel T. Chiu ; Richard H. Scheller ; Richard N. Zare ; Sandra E. Rodrí ; guez-Cruz ; Evan R. Williams ; Owe Orwar ; Mats Sandberg ; and J. Anders Lundqvist
• 刊名：Analytical Chemistry
• 出版年：1998
• 出版时间：September 1, 1998
• 年：1998
• 卷：70
• 期：17
• 页码：3517 - 3524
• 全文大小：128K
• 年卷期：v.70,no.17(September 1, 1998)
• ISSN：1520-6882

文摘

Several amine-containing components of individual vesiclesfrom the atrial gland of Aplysia californica were identified with capillary electrophoresis (CE). On-line derivatization with naphthalene-2,3-dicarboxaldehyde was performed, and the derivatized amine-containing componentswere detected with laser-induced fluorescence (LIF).Amino acids, including taurine, that had not been determined previously in atrial gland vesicles were observedby using CE-LIF, and their identities were confirmed withCE, HPLC, NMR, and electrospray ionization mass spectrometry. The finding that taurine is packaged and storedinto secretory vesicles supports the hypothesis that taurine may exhibit neuromodulatory activity. The bioactivepeptides, well-known to be in atrial gland vesicles, weredetected in lysed vesicle samples fractionated with HPLCand analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. These peptideswere also observed in single-vesicle runs with CE-LIF. Theatrial gland vesicles (ranging from 0.5 to 2 m diameterand 65 aL to 4 fL volume, respectively) studied in thiswork represent the smallest biological entities to beanalyzed chemically on an individual basis.