Electrochemical Analysis of Cell Plasma Membrane Cholesterol at the Airway Surface of Mouse Trachea
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- 作者：Dechen Jiang ; Danjun Fang ; Thomas J. Kelley ; James. D. Burgess
- 刊名：Analytical Chemistry
- 出版年：2008
- 出版时间：February 15, 2008
- 年：2008
- 卷：80
- 期：4
- 页码：1235 - 1239
- 全文大小：89K
- 年卷期：v.80,no.4(February 15, 2008)
- ISSN：1520-6882
文摘
Electrochemical detection of plasma membrane cholesterol at the surface of excised mouse trachea tissue isreported. Cholesterol oxidase is covalently linked to an11-mercaptoundecanoic acid submonolayer on the platinum electrode surface. The cholesterol oxidase-modifiedelectrodes show steady-state responses for cholesterol insolution at physiological temperatures. Experiments fordirect contact between the cholesterol oxidase-modifiedelectrode and the surface of excised trachea tissue at37 
C indicate steady-state responses that are largelyindependent of the position of contact on the tissuesurface. Tissue samples are mounted on a quartz crystalmicrobalance electrode to gauge contact force between theelectrode and the tissue surface, and the steady-stateelectrode response for tissue cholesterol is shown to belargely independent of the contact force. Trachea tissueexcised from a mouse model of cystic fibrosis, which isknown to exhibit evaluated cholesterol in airway cells,shows an electrode response that is ~40% larger than theresponse observed at wild-type mouse trachea tissue.
C indicate steady-state responses that are largelyindependent of the position of contact on the tissuesurface. Tissue samples are mounted on a quartz crystalmicrobalance electrode to gauge contact force between theelectrode and the tissue surface, and the steady-stateelectrode response for tissue cholesterol is shown to belargely independent of the contact force. Trachea tissueexcised from a mouse model of cystic fibrosis, which isknown to exhibit evaluated cholesterol in airway cells,shows an electrode response that is ~40% larger than theresponse observed at wild-type mouse trachea tissue.