Direct Electrochemical Evaluation of Plasma Membrane Cholesterol in Live Mammalian Cells
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- 作者：Dechen Jiang ; Anando Devadoss ; M. Simona Palencsá ; r ; Danjun Fang ; Nicole M. White ; Thomas J. Kelley ; Jonathan D. Smith ; James D. Burgess
- 刊名：Journal of the American Chemical Society
- 出版年：2007
- 出版时间：September 19, 2007
- 年：2007
- 卷：129
- 期：37
- 页码：11352 - 11353
- 全文大小：99K
- 年卷期：v.129,no.37(September 19, 2007)
- ISSN：1520-5126
文摘
Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.