Observation of Cellular Cholesterol Efflux at Microcavity Electrodes

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• 作者：Danjun Fang ; Dechen Jiang ; Hui Lu ; Hillel J. Chiel ; Thomas J. Kelley ; James D. Burgess
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：September 2, 2009
• 年：2009
• 卷：131
• 期：34
• 页码：12038-12039
• 全文大小：134K
• 年卷期：v.131,no.34(September 2, 2009)
• ISSN：1520-5126

文摘

Cholesterol oxidase modified platinum microcavity electrodes are used to measure cholesterol efflux from the plasma membrane surface of a single neuron in the buccal ganglion of Aplysia at room temperature. A background subtraction analog chronocoulometry method is used to measure hydrogen peroxide accumulation in the microcavity volume resulting from cellular cholesterol efflux and enzymatic oxidation. The data are consistent with the aqueous diffusion model for cellular cholesterol efflux where plasma membrane cholesterol undergoes exchange with solution phase cholesterol.