Amino-Modified Tetraphenylethene Derivatives as Nucleic Acid Stain: Relationship between the Structure and Sensitivity
详细信息 查看全文
- 作者：Li Xu ; Zece Zhu ; Danqing Wei ; Xiang Zhou ; Jingui Qin ; Chuluo Yang
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2014
- 出版时间：October 22, 2014
- 年：2014
- 卷：6
- 期：20
- 页码：18344-18351
- 全文大小：476K
- ISSN：1944-8252
文摘
A series of new amino-functionalized tetraphenylethene (TPE) derivatives were designed and synthesized to study the effect of molecular structures on the detection of nucleic acid. Contrastive studies revealed that the number of binding groups, the length of hydrophobic linking arm and the configuration of TPE molecule all play important roles on the sensitivity of the probes in nucleic acid detection. Z-TPE3 with two binding amino groups, long linking arms, and cis configuration was found to be the most sensitive dye in both solution and gel matrix. Z-TPE3 is able to stain dsDNA with the lowest amount of 1 ng and exclusively stain 40 ng of short oligonucleotide with only 10 nt. This work is of important significance for the further design of TPE probes as biosensors with higher sensitivity.