Salt-Leached Silk Scaffolds with Tunable Mechanical Properties

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• 作者：Danyu Yao ; Sen Dong ; Qiang Lu ; Xiao Hu ; David L Kaplan ; Bingbo Zhang ; Hesun Zhu
• 刊名：Biomacromolecules
• 出版年：2012
• 出版时间：November 12, 2012
• 年：2012
• 卷：13
• 期：11
• 页码：3723-3729
• 全文大小：655K
• 年卷期：v.13,no.11(November 12, 2012)
• ISSN：1526-4602

文摘

Substrate mechanical properties have remarkable influences on cell behavior and tissue regeneration. Although salt-leached silk scaffolds have been used in tissue engineering, applications in softer tissue regeneration can be encumbered with excessive stiffness. In the present study, silk-bound water interactions were regulated by controlling processing to allow the preparation of salt-leached porous scaffolds with tunable mechanical properties. Increasing silk-bound water interactions resulted in reduced silk II (尾-sheet crystal) formation during salt-leaching, which resulted in a modulus decrease in the scaffolds. The microstructures as well as degradation behavior were also changed, implying that this water control and salt-leaching approach can be used to achieve tunable mechanical properties. Considering the utility of silk in various fields of biomedicine, the results point to a new approach to generate silk scaffolds with controllable properties to better mimic soft tissues by combining scaffold preparation methods and silk self-assembly in aqueous solutions.