In-Situ Transmission Electron Microscopy of Liposomes in an Aqueous Environment

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• 作者：Sarah M. Hoppe ; Darryl Y. Sasaki ; Aubrianna N. Kinghorn ; Khalid Hattar
• 刊名：Langmuir
• 出版年：2013
• 出版时间：August 13, 2013
• 年：2013
• 卷：29
• 期：32
• 页码：9958-9961
• 全文大小：250K
• 年卷期：v.29,no.32(August 13, 2013)
• ISSN：1520-5827

文摘

The characterization of liposomes was undertaken using in-situ microfluidic transmission electron microscopy. Liposomes were imaged without contrast enhancement staining or cryogenic treatment, allowing for the observation of functional liposomes in an aqueous environment. The stability and quality of the liposome structures observed were found to be highly dependent on the surface and liposome chemistries within the liquid cell. The successful imaging of liposomes suggests the potential for the extension of in-situ microfluidic TEM to a wide variety of other biological and soft matter systems and processes.