Nanoscale Patterning of Membrane-Bound Proteins Formed through Curvature-Induced Partitioning of Phase-Specific Receptor Lipids

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• 作者：Maria O. Ogunyankin ; Dale L. Huber ; Darryl Y. Sasaki ; Marjorie L. Longo
• 刊名：Langmuir
• 出版年：2013
• 出版时间：May 21, 2013
• 年：2013
• 卷：29
• 期：20
• 页码：6109-6115
• 全文大小：470K
• 年卷期：v.29,no.20(May 21, 2013)
• ISSN：1520-5827

文摘

This work describes a technique for forming high-density arrays and patterns of membrane-bound proteins through binding to a curvature-organized compositional pattern of metal-chelating lipids (Cu²⁺-DOIDA or Cu²⁺-DSIDA). In this bottom-up approach, the underlying support is an e-beam formed, square lattice pattern of hemispheres. This curvature pattern sorts Cu²⁺-DOIDA to the 200 nm hemispherical lattice sites of a 600 nm 脳 600 nm unit cell in L_d - L_o phase separated lipid multibilayers. Binding of histidine-tagged green fluorescent protein (His-GFP) creates a high density array of His-GFP-bound pixels localized to the square lattice sites. In comparison, the negative pixel pattern is created by sorting Cu²⁺-DSIDA in L_d - L_{尾鈥?/sub> phase separated lipid multibilayers to the flat grid between the lattice sites followed by binding to His-GFP. Lattice defects in the His-GFP pattern lead to interesting features such as pattern circularity. We also observe defect-free arrays of His-GFP that demonstrate perfect arrays can be formed by this method suggesting the possibility of using this approach for the localization of various active molecules to form protein, DNA, or optically active molecular arrays.}