Microbial De-emulsification: A Highly Efficient Procedure for the Extractive Workup of Whole-Cell Biotransformations

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• 作者：Kathrin Leppchen ; Thomas Daussmann ; Simon Curvers ; Martin Bertau
• 刊名：Organic Process Research & Development
• 出版年：2006
• 出版时间：November 2006
• 年：2006
• 卷：10
• 期：6
• 页码：1119 - 1125
• 全文大小：158K
• 年卷期：v.10,no.6(November 2006)
• ISSN：1520-586X

文摘

Formation of stable emulsions with the organic solvent is ageneral complication in the extractive workup of aqueous whole-cell biotransformations. This hold-up has been overcome bybiocatalytic lysis of emulsifying agents present in the mediumthrough addition of living microorganisms. Of these, Bacillussubtilis and Rhodococcus erythropolis exhibited the most powerful de-emulsifying activity. As exemplified by microbial treatment of cell-free biotransformation media of Saccharomycescerevisiae and Lactobacillus kefiri, phase separation time (t_p)was drastically reduced from one week to 20 s withoutsignificantly affecting product integrity. This practicable ready-to-use method is appropriate to both fungal and bacterialbiocatalysts. The highly efficient de-emulsification power andthe considerably short phase separation time of this techniqueallow for cost-effective continuous extractions on a large-scale,for example with mixer-settler units.