Study on Mesophilic and Thermophilic Alcohol Dehydrogenases in Gas-Phase Reaction
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- 作者：Archana H. Trivedi ; Antje C. Spiess ; Thomas Daussmann ; and Jochen Bü ; chs
- 刊名：Biotechnology Progress
- 出版年：2006
- 出版时间：April 2006
- 年：2006
- 卷：22
- 期：2
- 页码：454 - 458
- 全文大小：64K
- 年卷期：v.22,no.2(April 2006)
- ISSN：1520-6033
文摘
The initial reaction rate and the thermostability of the mesophilic alcohol dehydrogenase (ADH)from Lactobacillus brevis (LBADH), and the thermophilic ADH from Thermoanaerobacter sp.(ADH T) in gas-phase reaction were compared. The effects of water activity, cofactor-to-proteinmolar ratio, and reaction temperature on the reduction of acetophenone to 1-phenylethanol werestudied. An optimal water activity of 0.55 in terms of productivity was found for both ADHs.The cofactor-to-protein molar ratio was chosen slightly higher than equimolar to increase bothactivity and thermostability. An excellent optimal productivity of 1000 g·L-1·d-1 for LBADHand 600 g·L-1·d-1for ADH T was found at 60 
C, while the highest total turnover numberswith respect to the enzyme were achieved at 30 C and amounted to 4.2 million for LBADHand 1.7 million for ADH T, respectively. Interestingly, the ADH from the mesophilic L. brevisshowed the higher thermostability in the nonconventional medium gas phase.
C, while the highest total turnover numberswith respect to the enzyme were achieved at 30 C and amounted to 4.2 million for LBADHand 1.7 million for ADH T, respectively. Interestingly, the ADH from the mesophilic L. brevisshowed the higher thermostability in the nonconventional medium gas phase.