Functional Delivery of siRNA by Disulfide-Constrained Cyclic Amphipathic Peptides
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- 作者：Jade J. Welch ; Ria J. Swanekamp ; Christiaan King ; David A. Dean ; Bradley L. Nilsson
- 刊名：ACS Medicinal Chemistry Letters
- 出版年：2016
- 出版时间：June 9, 2016
- 年：2016
- 卷：7
- 期：6
- 页码：584-589
- 全文大小：353K
- 年卷期：0
- ISSN：1948-5875
文摘
The promise of oligonucleotide therapeutic agents to perturb expression of disease-related genes remains unrealized, in part due to challenges with functional cellular delivery of these agents. Herein, we describe disulfide-constrained cyclic amphipathic peptides that complex with short-interfering RNA (siRNA) and affect functional cytosolic delivery and knockdown of target gene products in cell culture and in vivo to mouse lung. Reduction of the constraining disulfide bond and subsequent proteolytic clearance of the peptide are key design features that allow unmasking of the siRNA cargo and presentation to the RNA interference machinery.