Construction of a 4 Zeptoliters Switchable 3D DNA Box Origami
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- 作者：Reza M. Zadegan ; Mette D. E. Jepsen ; Karen E. Thomsen ; Anders H. Okholm ; David H. Schaffert ; Ebbe S. Andersen ; Victoria Birkedal ; J酶rgen Kjems
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：November 27, 2012
- 年：2012
- 卷：6
- 期：11
- 页码：10050-10053
- 全文大小：301K
- 年卷期：v.6,no.11(November 27, 2012)
- ISSN：1936-086X
文摘
The DNA origami technique is a recently developed self-assembly method that allows construction of 3D objects at the nanoscale for various applications. In the current study we report the production of a 18 脳 18 脳 24 nm3 hollow DNA box origami structure with a switchable lid. The structure was efficiently produced and characterized by atomic force microscopy, transmission electron microscopy, and F枚rster resonance energy transfer spectroscopy. The DNA box has a unique reclosing mechanism, which enables it to repeatedly open and close in response to a unique set of DNA keys. This DNA device can potentially be used for a broad range of applications such as controlling the function of single molecules, controlled drug delivery, and molecular computing.