Surface Chemistry of Bacteriophage and Laser Ablated Nanoparticle Complexes for Pathogen Detection

详细信息    查看全文

• 作者：Nancy Tawil ; Edward Sacher ; David Rioux ; Rosemonde Mandeville ; Michel Meunier
• 刊名：Journal of Physical Chemistry C
• 出版年：2015
• 出版时间：June 25, 2015
• 年：2015
• 卷：119
• 期：25
• 页码：14375-14382
• 全文大小：360K
• ISSN：1932-7455

文摘

We report the synthesis of colloidal nanoparticles, produced by the femtosecond laser ablation of a gold target, and their subsequent use in the formation of S. aureus phage鈥搉anoparticle complexes for biodetection purposes. A detailed X-ray photoelectron spectroscopic analysis shows that the negatively charged nanoparticles that are formed interact with and subsequently internalize into the positively charged bacteriophages. These complexes render possible the specific detection of a single S. aureus bacterium in a heterogeneous sample, using dark field microscopy without complex sample treatment or amplification.