Biological Insights from a Simulation Model of the Critical FtsZ Accumulation Required for Prokaryotic Cell Division

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• 作者：Claire E. Dow ; Hugo A. van den Berg ; David I. Roper ; Alison Rodger
• 刊名：Biochemistry
• 出版年：2015
• 出版时间：June 23, 2015
• 年：2015
• 卷：54
• 期：24
• 页码：3803-3813
• 全文大小：417K
• ISSN：1520-4995

文摘

A simulation model of prokaryotic Z-ring assembly, based on the observed behavior of FtsZ in vitro as well as on in vivo parameters, is used to integrate critical processes in cell division. According to the model, the cell鈥檚 ability to divide depends on a 鈥渃ontraction parameter鈥?(蠂) that links the force of contraction to the dynamics of FtsZ. This parameter accurately predicts the outcome of division. Evaluating the GTP binding strength, the FtsZ polymerization rate, and the intrinsic GTP hydrolysis/dissociation activity, we find that inhibition of GTP鈥揊tsZ binding is an inefficient antibacterial target. Furthermore, simulations indicate that the temperature sensitivity of the ftsZ84 mutation arises from the conversion of FtsZ to a dual-specificity NTPase. Finally, the sensitivity to temperature of the rate of ATP hydrolysis, over the critical temperature range, leads us to conclude that the ftsZ84 mutation affects the turnover rate of the Z-ring much less strongly than previously reported.