Utility of Metabolomics toward Assessing the Metabolic Basis of Quality Traits in Apple Fruit with an Emphasis on Antioxidants
详细信息 查看全文
- 作者：Daniel Cuthbertson ; Preston K. Andrews ; John P. Reganold ; Neal M. Davies ; B. Markus Lange
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：September 5, 2012
- 年：2012
- 卷：60
- 期：35
- 页码：8552-8560
- 全文大小：394K
- 年卷期：v.60,no.35(September 5, 2012)
- ISSN：1520-5118
文摘
A gas chromatography鈥搈ass spectrometry approach was employed to evaluate the use of metabolite patterns to differentiate fruit from six commercially grown apple cultivars harvested in 2008. Principal component analysis (PCA) of apple fruit peel and flesh data indicated that individual cultivar replicates clustered together and were separated from all other cultivar samples. An independent metabolomics investigation with fruit harvested in 2003 confirmed the separate clustering of fruit from different cultivars. Further evidence for cultivar separation was obtained using a hierarchical clustering analysis. An evaluation of PCA component loadings revealed specific metabolite classes that contributed the most to each principal component, whereas a correlation analysis demonstrated that specific metabolites correlate directly with quality traits such as antioxidant activity, total phenolics, and total anthocyanins, which are important parameters in the selection of breeding germplasm. These data sets lay the foundation for elucidating the metabolic basis of commercially important fruit quality traits.