Efficient Primer Strand Extension beyond Oxadiazole Carboxamide Nucleobases

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• 作者：Olga Adelfinskaya ; Vishal C. Nashine ; Donald E. Bergstrom ; and V. Jo Davisson
• 刊名：Journal of the American Chemical Society
• 出版年：2005
• 出版时间：November 23, 2005
• 年：2005
• 卷：127
• 期：46
• 页码：16000 - 16001
• 全文大小：35K
• 年卷期：v.127,no.46(November 23, 2005)
• ISSN：1520-5126

文摘

Two oxadiazole carboxamide deoxyribonucleoside analogues are described that can be incorporated and efficiently extended by Taq DNA polymerase. The primer strand extension beyond oxadiazole nucleoside analogues occurs at rates similar to the values observed for the canonical Watson-Crick base pairs irrespective of the template nucleobase. These distinctive chemical effects in DNA polymerase extensions are attributed to the smaller size and unique electronic properties of the oxadiazole nucleobase.